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Phosphate Binding in Wild Type TIGAR Two phosphate ions are found in the active site of full length TIGAR. The first phosphate ion is coordinated by His11, His183, Arg10, Arg61 and Glu89. Second phosphate ion is coordinated by Arg10 and Asn217. Fru-1,6-bisphosphate and Fru-2,6-bisphosphate can be placed in the position of the two phosphate molecules. Described
amino acids are shown in sticks view in yellow and blue color.
Bound phosphate ions are shown in space-filling view. Backbone
is shown in trace view.
Hua Li and Gerwald Jogl
Structural and Biochemical Studies of TIGAR(TP53-induced Glycolysis and Apoptosis Regulator The Journal of Biological Chemistry, 284,1748-1754 (PubMed) 3E9D (PDB) |
Phosphate Binding in
H11A Mutant TIGAR Mutation of Histidine 11 to Alanine did not abrogate phosphate binding in active site. The binding of phosphate ion was shown to shift towards the position of absent His11 side chain. Phosphate ion was shown to have additional interaction with water molecule (WE393) that occupies the position of absent Histidine side chain. Described
amino acids are shown in sticks view in yellow and blue color.
Bound phosphate ions and water molecule are shown in
space-filling view.Backbone is shown in trace view.
Hua Li and Gerwald Jogl
Structural and Biochemical Studies of TIGAR(TP53-induced Glycolysis and Apoptosis Regulator The Journal of Biological Chemistry, 284,1748-1754 (PubMed) 3E9E (PDB) |